Thursday 21 July 2011

Peqlab EVOS digital fluorescent microscope

A microscope without eye pieces? Crazy?!! Not really, actually this one is pretty neat I think. We had the EVOS fl in for half a day on a demo and its really good. Nice clear images of fluorescently labelled cells acquired in a pretty short time. You can do at least 3 colours and have a phase contrast image. It uses essentially Olympus lenses that range from 2x-40x with no oil. And there is a 100x/ oil immersion available too.
It struck me as being a really good system for checking your GFP transfections, or screening your IF slides before maybe going onto taking images on more sophisticated systems. As there are no eye pieces your images are displayed on a 12" TFT screen for the world to see! Makes a good teaching tool I think, easy to explain things to students without trying to get them to look down the microscope! I guess it could be tricky to find the elusive transfected cell on a coverslip that maybe doesn't contain a great deal of them - but then that's always tricky!
I'll try and get a few images from people as examples, but for now a few iPhone snap shots:
EVOS fl, multicolour fluorescence with no eyepieces!!
The brightfield version with a colour camera

A range of lenses

2 comments:

  1. Well if you ever get tired of a microscope without the essential microscope parts you should definitely check this website out. Just bought a brand new bulb from them and they were fantastic.

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  2. Good job! I loved it and continue your passion.

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